In the short term, islet transplant recipients do well. However, longer term, we see cases of rejection increase.Transplant rejection is a major cause of graft loss and loss of function. It is important that we develop a means of predicting the likelihood of rejection, so that we can intervene.We built on existing research and developed a consistent method of using flow cytometry to analyze the immune-cell-count, and subsets of immune cells present in the blood of transplant recipients. This involved the standardization of a number of factors, such as sample handling and instrument set up.We found that this method was easy to use and produced consistent results across a number of samples.Importantly, we also found that this method had a very limited impact on the patient – only 1.5mL of blood was required for testing.” Reviewed by Kate Anderton, B.Sc. (Editor)May 29 2019Researchers from The Westmead Institute for Medical Research have developed a standardized method of measuring the immune response in islet transplant recipients, helping predict patient outcomes.Islet transplantation is a frontier therapy for type 1 diabetes with notable success in hypoglycaemic patients, where pancreatic beta cells from a donor are transplanted into a recipient, enabling the body to produce insulin, and regulate blood sugar.Transplant recipients are given immunosuppressant medication to prevent the immune system from attacking and destroying the transplant. However, rejection may still occur in certain instances.Lead researcher Dr Min Hu and her team aimed to standardize a method using flow cytometry – technology that detects and measures the characteristics of a group of cells – to analyze the immune cells present in blood samples.The research built on existing findings, which established that certain profiling panels (sets of characteristics that are detected and measured) could be used on flow cytometers to monitor the major immune cells involved in transplantation.Dr Hu said: Related StoriesComplement system shown to remove dead cells in retinitis pigmentosa, contradicting previous researchOne-fifth of U.S. surgeons still ‘overusing’ riskier method to create kidney dialysis access, study findsFight for Sight poll: Brits put their eyesight at risk through unsafe contact lens habitsIslet transplantation is currently only administered in clinical trials. Dr Hu said the research findings could be used in conjunction with current clinical trials to monitor patients after a transplant.”Islet transplantation is an important breakthrough for people with type 1 diabetes. If the transplant is tolerated, it can significantly improve the patients’ quality of life.”Unfortunately, we currently have no method of predicting how a patient will respond to a transplant.”By using this standardized method of flow cytometry to monitor changes in the immune system, this research could help predict whether a patient will reject a transplant, enabling early intervention, and the potential development of other solutions to stop rejection from occurring.”The paper was published in PLOS ONE.Source:Westmead Institute for Medical ResearchJournal reference:Hu, M. et al. (2019) Standardisation of flow cytometry for whole blood immunophenotyping of islet transplant and transplant clinical trial recipients. PLOS ONE. doi.org/10.1371/journal.pone.0217163.
Published on Alleges ₹11-lakh cr scam; Left, Trinamool back all-India protest SHARE politics September 06, 2018 COMMENTS SHARE SHARE EMAIL COMMENT The Congress has called for a Bharat Bandh on Monday alleging that the rising prices of petroleum products is a scam worth ₹11-lakh crore, while also inviting other Opposition parties to join the protest. The Left parties have backed the strike-call, and the Trinamool Congress, while backing the protests, said it would not take part in the bandh.The Congress’ decision came after a meeting of its general secretaries, State in-charges and State presidents here on Thursday. Senior leaders Ahmed Patel, Ashok Gehlot and Mallikarjun Kharge have been asked to reach out to other political parties. Talking to reporters, Patel said he was hopeful that all political parties would support the strike call. He said the prices of fuel had reached a point whereby it was necessary for the Opposition to ventilate the people’s feelings.The Congress alleged that the rise in prices is a scam worth ₹11-lakh crore. “On May 16, 2014, price of petrol was ₹51.78 per litre. Today it is ₹79.51 per litre. Price of diesel in 2014 was ₹44 per litre. Today it is ₹71 per litre in Delhi. A RTI query has revealed that the Modi government is selling diesel for ₹34 per litre and petrol for ₹37 per litre to other countries, whereas in India the price is too high,” Congress’s media in-charge, Randeep Singh Surjewala, said.He said the excise duty on petrol, which was ₹9 per litre in May 2014, is currently ₹19 per litre. “The excise duty on diesel in 2014 was ₹3 and today it is approximately ₹15. PM Modi has collected ₹11,00,000 crore by this hike. Where has the money gone?” Surjewala asked.The party also pointed to the increase in the price of LPG from ₹400 to ₹800. Prices of items of daily use have been inflated by the Modi government. Gehlot said the parties will also hold protest sit-ins in front of petrol pumps. “The States and the Centre had reduced the taxes on petroleum products during the UPA’s time. Now, the Centre is not ready to forego taxes on petroleum products,” he said.Gehlot added that the bandh will be from 9 am to 3 pm on Monday so that the common people are not inconvenienced. The Congress has also been demanding that the petroleum prices should be brought under the ambit of GST. The regional and Left parties, however, are opposed to this decision. read more
CHENNAI: With Isro yet to officially announce the nature of the technical snag that prompted it to call off launch of Chandrayaan 2, a former space scientist Tuesday commended the team for halting the high-stake Moon mission. The launch of India’s second Moon mission aimed at landing a rover on the unexplored Lunar South Pole was aborted early Monday due to a “technical snag” minutes before the scheduled lift-off of the country’s most powerful rocket GSLV-MKIII from the spaceport of Sriharikota. Former Indian Space Research Organisation (ISRO) scientist Nambi Narayanan, credited for his contributions in the development of cryogenic technology in the country’s space programme, said calling off the launch was disappointing not just for the general public but even for the brains behind the Rs 978 crore project. “It is a pity that countdown was stopped. They (scientists) might have noticed something wrong. I feel the problem might (have been even) small but they must not have wanted to take a chance”, he told PTI. Narayanan’s comments came even as the Isro was yet to make any formal announcement on what went wrong during the final hour of countdown that prompted the space agency to call it off 56 minutes before the lift-off. Though none of the Isro officials whom PTI contacted responded, experts have said a glitch might have been noticed when the rocket’s liquid propellant was being loaded into the cryogenic upper stage engine. Incidentally, an update by Isro at around 1.34 AM Monday had said “Filling of Liquid Hydrogen in Cryogenic stage of #GSLVMkIII-M1 completed. #Chandrayaan2 #ISRO Stay tuned for more updates..” Narayanan said it was good ISRO halted the launch after noticing the glitch. “It may be a small thing, but that is always good (halting a launch instead of going ahead). They are now trying to understand what went wrong. what actually happened. So, we need to wait..” he said. Isro had earlier scheduled the launch of the mission, headed by women scientists Ritu Karidhal and Muthayya Vanitha, in the first week of January but shifted it to July 15 after choosing a launch window between July 9 and 16. Chandrayaan-2, comprising an orbiter, a lander and a rover, was to have been injected into an Earth orbit about 16 minutes after lift-off with the lander to touch down on the Moon surface 54 days later through a series of critical orbit rising manoeuvres. Download The Times of India News App for Latest India News.XStart your day smart with stories curated specially for you read more